Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori

Abstract Background 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition different insects. In Bombyx mori , is induced by 20E larval fat body. Long non-coding RNAs (lncRNAs) function various biological processes many organisms, including Many lncRNAs have reported to be potential for occurrence mammals, but it not investigated Results RNA libraries from body of B. dissected at 2 6 h post-injection with were constructed sequenced, comprehensive analysis mRNAs was performed. A total 1035 identified, 905 lincRNAs 130 antisense lncRNAs. Compared mRNAs, had longer transcript length fewer exons. 132 found differentially expressed post injection, compared 64 injection. Thirty common post-injection, hypothesized associated response. Target gene predicted 6493 lncRNA-mRNA cis pairs 42,797 trans pairs. The expression profiles LNC_000560 highly consistent its target genes, Atg4B RNAi significantly decreased . These results indicated that potentially involved regulating Conclusions This study provides genome-wide identification functional characterization identified related 20-regulated will helpful further studying regulatory mechanisms other this model.